USE PRINCIPLES
The Nodularin plate kit is a competitive enzyme-labeled immunoassay. The Nodularin HRP conjugate, sample extract, and calibrators are pipetted into the test wells followed by the Nodularin antibody into the test wells to initiate the reaction. During the 45-minute incubation period, Nodularin from the sample and Nodularin HRP conjugate compete for binding to the Nodularin antibody. The Nodularin antibody is captured on the walls of the test well. Following this 45-minute incubation, the contents of the well are removed, and the wells are washed to remove any unbound Nodularin, Nodularin HRP conjugate, and free Nodularin antibody. After wash, a clear substrate is added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 45-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Nodularin concentration of the samples is derived.

PRECAUTIONS
•   Store all kit components at 4°C to 8°C (39°F to 46°F) when not in use.
•   Each reagent is optimized for use in the Nodularin Plate Kit. Do not substitute reagents from any other manufacturer into the test kit. Do not combine reagents from other Nodularin Plate Kits with different Lot numbers.
•   Dilution or adulteration of reagents or samples not called for in the procedure may produce inaccurate results.
•   Do not use reagents after the expiration date.
•   Reagents should be brought to room temperature, 20 – 28ºC (62 – 82ºF) prior to use. Avoid prolonged (> 24 hours) storage at room temperature.
•   Nodularin is a toxin and should be treated with care.
•   The Stop Solution is 1N hydrochloric acid. Avoid contact with skin and mucous membranes. Immediately clean up any spills and wash the area with copious amounts of water. If contact should occur, immediately flush with copious amounts of water.
•   Transfer of samples and reagents by pipette requires constant monitoring of the technique. Pipetting errors are the major source of error in immunoassay methodology.