INTENDED USE
The Progesterone Plate Kit is an immunological laboratory test for the quantitation of Progesterone in dairy products such as milk, cheese, yogurt, cream, ice cream and butter.
REAGENTS AND MATERIALS PROVIDED
The kit in its original packaging can be used until the end of the month indicated on the box label when stored at 2 – 8ºC.
• 1 frame containing 12 test strips of 8 wells each, vacuum-packed in aluminized pouch with indicating desiccant.
• 4 vials each containing 4 mL Progesterone calibrators corresponding to 0, 0.1, 1 and 10 μg/L (ppb) Progesterone.
• 1 vial of 8 mL Progesterone-HRP Enzyme Conjugate.
• 1 vial of 8 mL Rabbit anti-Progesterone antibody.
• 1 vial containing 14 mL of Substrate.
• 1 vial containing 14 mL of Stop Solution. (Caution! 1N HCl.)
• 1 vial containing 8 mL of 1N HCl for extraction. (Handle with care!)
• 1 vial containing 50 mL of Wash Solution concentrate (10X).
ASSAY PRINCIPLES
The Progesterone Plate Kit is a competitive enzyme-labeled immunoassay. Progesterone is extracted from dairy product samples such as milk, cheese, yogurt or cream, etc. The extract is diluted and tested in the immunoassay. Progesterone-enzyme conjugate is pipetted into the test wells followed by calibrators or sample extracts. Progesterone antibody is then pipetted into the test wells to initiate the reaction.
During the 30-minute incubation period, Progesterone from the sample and Progesterone-enzyme conjugate compete for binding to Progesterone antibody which, in turn, binds to the test well. Following this 30-minute incubation, the contents of the well are removed, and the wells are washed to remove any unbound compounds or enzyme-labeled compounds. A clear substrate is then added to the wells and any bound Progesterone-enzyme conjugate causes the conversion to a blue color. Following a 30-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the Progesterone concentration of the samples is derived.
| Format: | 96-well microtiter plate (12 test strips of 8 wells) |
|---|---|
| Standards: | 0, 0.1, 1, 10 ppb |
| Incubation Time: | 60 minutes |
There are no reviews yet.
Only logged in customers who have purchased this product may leave a review.
