INTENDED USE
The Melamine Plate Kit is a competitive ELISA for the quantitative analysis of Melamine in contaminated samples.
MATERIALS REQUIRED BUT NOT PROVIDED
• Laboratory quality distilled or deionized water.
• Pipet with disposable tips capable of dispensing 50 µL.
• Multi-channel pipet; 8 channel capable of dispensing 50 and 100 µL.
• Paper towels or equivalent absorbent material.
• Microwell plate or strip reader with 450nm filter.
• Timer
• Wash solution and bottle. PBST wash solution:10mM PBS + 0.05% Tween 20, pH 7.4.
ASSAY PRINCIPLES
The Melamine plate kit is a competitive enzyme-labeled immunoassay. Melamine is extracted from a sample by blending or shaking with extraction solution. The Melamine HRP conjugate, sample extract and calibrators are pipetted into the test wells coated with Melamine antibody to initiate the reaction.
During the 30-minute incubation period, Melamine from the sample and Melamine HRP conjugate compete for binding to Melamine antibody. Following this 30-minute incubation, the contents of the well are removed, and the wells are washed to remove any unbound Melamine, and Melamine HRP conjugate. After wash with laboratory grade water, a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 30-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Melamine concentration of the samples is derived.
| Format: | 96-well microtiter plate (12 test strips of 8 wells) |
|---|---|
| Standards: | 0, 20, 100, 500 ppb |
| Incubation Time: | 60 minutes |
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