Plasmid maxiprep columns are supplied with spin columns and collection tubes. The columns are based on silica membrane technology and provide fast and reliable extraction of high-quality plasmid with a binding capacity of 500 μg (NP500) or 1.5 mg (HP1000-P).
Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.
Features
Pack Size:
Order information:
| Cat. # | Description | Volume | Yield | Qty. |
|---|---|---|---|---|
| NP500 | Plasmid Maxiprep Columns | 50 mL，18 mL | ~500 μg | 20/Box |
| HP1000-P | High-yield Plasmid Maxiprep Columns | 50 mL，18 mL | ~1.5 mg | 20/Box |
| MNP001-4 | Plasmid Maxiprep Kits | 1 | 1 | 10 Preps |
Note: It is recommended that the bacteria culture should be 100-200 mL and the elution volume should be 1.0-2.0 mL by using Plasmid Maxiprep Columns.
Experimental overview
Typical results
Table 1: The yield of plasmid DNA extracted using various columns
| 1 | A260/280 | Concentration（ng/μL） | Yield（μg） |
| NP500 | 1.95 | 300 | 300 |
| 1 | 1.91 | 337 | 320 |
| HP1000-P | 2.01 | 780 | 780 |
| 1 | 1.98 | 800 | 800 |
pUC19 was extracted from 120 mL overnight E. coli (DH5a) using columns NP500 HP1000, and the elution volume was 1.0 ml.
| Product No | HP1000-P, MNP001-4, NP500 |
|---|---|
| Packing Size | 20 pcs/pack |
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