Plasmid miniprep columns are supplied with spin columns and collection tubes. The columns are based on silica membrane technology and provide fast and reliable extraction of high-quality plasmid with a binding capacity of 30μg.
Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.
Features
Pack Size:
Order information:
| Cat. # | Description | Volume | Yield | Qty. |
|---|---|---|---|---|
| NP30 | Plasmid Miniprep Columns,Capless spin columns, Blue fixing rings | 2 mL，800 μL | ~30 μg | 500/Box |
| NP30-A | Plasmid Miniprep Columns,Capped spin columns, Blue fixing rings | 2 mL，800 μL | ~30 μg | 500/Box |
| NP30-AS | Plasmid Miniprep Columns, Capped spin columns, White fixing rings | 2 mL，750 μL | ~30 μg | 500/Box |
| MNP001-1 | Plasmid Miniprep Kit | 50 Preps | ||
| MNP016-1 | Rapid Plasmid Miniprep Kit | 50 Preps | ||
Note: It is recommended that the elution volume should be 60-100 μL by using a series of BeingBio NP30.
Experimental overview
Typical results
pUC19 was extracted from 3 mL overnight E. coli (DH5a) using columns, and the elution volume was 80 μL. Meanwhile, extracted plasmid pUC19 from 10 mL overnight E. coli (DH5a) using BeingBio HP50, and the elution volume was 100 μL. Then analyze by electrophoresis on a 1% agarose gel.
Lane 1: Column from Company A
Lane 2: Column from company T
Lane 3: BeingBio NP30
Lane 4: BeingBio HP50
Figure 1: Plasmid DNA extracted using various columns
Table 1: The yield of plasmid DNA extracted using various columns
| A260/280 | Concentration (ng/μL) | Yield (μg) | |
|---|---|---|---|
| Column from company A | 1.92 | 312 | 25 |
| 1.96 | 300 | 24 | |
| Column from company T | 1.86 | 350 | 28 |
| 1.82 | 325 | 26 | |
| BeingBio NP30 | 1.91 | 350 | 28 |
| 1.88 | 337 | 27 | |
| BeingBio HP50 | 2.01 | 800 | 80 |
| 2.03 | 850 | 85 |
| Product No | MNP001-1, MNP016-1, NP30, NP30-A, NP30-AS |
|---|---|
| Packing Size | 50 Preps, 500 pcs/box |
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