ASSAY PRINCIPLES
The Diamino Atrazine Tube Kit uses polyclonal antibodies that bind both diamino Atrazine and a Diamino Atrazine-enzyme conjugate. Diamino atrazine in the sample compete with diamino atrazine-enzyme conjugate for a limited number of antibody binding sites. Antibodies, which bind Diamino Atrazine, are immobilized to the inside of the test tubes.

In the assay procedure you will:
•   Add samples and calibrators containing known amounts of Diamino Atrazine and a Diamino Atrazine enzyme conjugate to test tubes coated with anti-Diamino Atrazine antibodies. The conjugate competes with any Diamino Atrazine in the sample for the same antibody binding sites.
•   Wash away any unbound molecules, after you incubate this mixture for 60 minutes.
•   Add clear substrate solution to each tube. In the presence of bound Diamino Atrazine-enzyme conjugate, the substrate is converted to a blue compound. One enzyme molecule can convert many substrate molecules.

Since the same number of antibody binding sites are available in every tube, and each tube receives the same number of Diamino Atrazine-enzyme conjugate molecules, a sample containing a low concentration of Diamino Atrazine allows the antibody to bind many Diamino Atrazine-enzyme conjugate molecules. The result is a dark blue solution. Conversely, a high concentration of Diamino Atrazine allows fewer Diamino Atrazine-enzyme conjugate molecules to be bound by the antibodies, resulting in a lighter blue solution.

NOTE: Color is inversely proportional to Diamino Atrazine concentration. Therefore:
Darker color = Lower concentration
Lighter color = Higher concentration