ASSAY PRINCIPLES
Monkeypox Virus IgM/IgG Ab Test uses colloidal gold labeled monkeypox recombinant antigen and mouse IgG, with nitrocellulose membrane fixed with two detection lines (T1 and T2) and a quality control line (C line). The T1 line was fixed with mouse anti-human IgM antibody and used to detect monkeypox IgM antibody. The mouse anti-human IgG antibody was fixed on the T2 line to detect monkeypox IgG antibodies. The sheep anti-mouse IgG antibody was fixed inline C. When adding a suitable amount of sample in the sample hole, samples will be along the strip under the capillary action to move forward, if the sample contains monkeypox IgM antibody, monkeypox IgM antibody will first with colloidal gold labeled monkeypox recombinant antigen to form a complex, this complex will be membrane on the mouse anti-human IgM antibody capture, a red strip appears on the detection line T1, Indicating monkeypox IgM antibody positive; If the sample contains monkeypox IgG antibody, monkeypox IgG antibody will first with colloidal gold labeled monkeypox recombinant antigen to form a complex, this complex will be membrane on the mouse anti-human IgG antibody capture, a red strip appears on the detection line T2, Indicating monkeypox IgG antibody positive. Neither T1 nor T2 lines appear, indicating a negative result. Whether or not Monkeypox Virus IgM/IgG antibodies are present in the sample, a red band is formed in the quality control area (C). The color of the quality control area (C) is the standard to determine whether
there are enough samples and whether the chromatography process is normal, and it also serves as the internal control standard of the reagent.