INTENDED USE

CommaX^TM His gravity flow columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni²⁺ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with poly-histidine tags.

Recombinant proteins with poly-histidine tags are retained due to the formation of complex compounds between their poly-histidine tags and immobilized Ni²⁺ ions. Impurity proteins without poly-histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

CommaX^TM His gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.

FEATURES

• Puncturable upper caps confer easy connection with peristaltic pumps and syringes
• Ease of use, no need for dedicated purification systems

APPLICATIONS

• Purification of recombinant proteins with poly-histidine tags from various express systems, such as bacterial, insect and mammal cells.

INTENDED USE

His-Tag Purification prepacked columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni²⁺ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with Poly histidine tags. The Beingbio His prepacked columns provide a fast, convenient, and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.

Recombinant proteins with Poly histidine tags are retained due to the formation of complex compounds between their Poly histidine tags and immobilized Ni²⁺ ions. Impurity proteins without Poly histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

His-Tag Purification prepacked columns are available in 1 mL and 5 mL sizes.

FEATURES

• Superior biocompatibility
• Uncompromised biological activities
• High purification yields
• Moderate experimental conditions

APPLICATIONS

• Purification of recombinant proteins with Poly-histidine tags from various express systems, such as bacterial, insect, and mammal cells.
• purification of His tagged membrane proteins to study lipid binding.
• purification of His tagged spider toxin and variants to evaluate sodium channel inhibition.

0.65ml, 1.5 ml & 2.0 ml Vials

• Non-skid rubber feet hold the rack in place.
• Supports up to 48 tubes in an alpha-numeric configuration.
• Manufactured from 100% recycled polypropylene.
• Certified RNase/DNase Free
  Certified PCR Inhibitor Free
  Certified Human DNA Free
  Certified Pyrogen Free

Intended Use
Mini Plasmid Purification Kit （Magnetic Beads ）lyses cells by alkaline lysis, and then adsorbs and releases plasmid DNA by silica-based magnetic beads, so as to separate and purify plasmid DNA. The purified plasmid DNA is suitable for downstream applications such as restriction digestion, PCR amplification, library reparation and other molecular biology experiments. Magnetic bead method is not only more convenient and faster to extract plasmid DNA, but also can be used with various nucleic acid extraction equipment or workstation.

Features

♦ Kit can quickly extract and purify plasmid DNA without centrifugation.
♦ The buffer solution system exclude Phenol/chloroform, it is safe to operator and environment.
♦ The kit can be used with nucleic acid extraction instruments to get large-scale and high-throughput extraction. It is simple, fast and efficient.

BeingBio’s Real-time PCR System is engineered to advance molecular diagnostics through precision, speed, and innovation, and is designed to streamline and accelerate the research and testing processes. The product is combined with PCR quantitative detection kit with fluorescent marker, which is used for rapid amplification and qualitative and quantitative analysis of nucleic acid specific fragments. Its core strengths lie in its smart operational features, simplifying the user experience. this system delivers fast, reliable results, making it an indispensable tool in laboratories.

The system is designed for high throughput with minimal well-to-well and instrument variation, ensuring reliable and reproducible results. Compatibility with millions of assays underlines its versatility in molecular biology diagnostics. Its compact design integrates seamlessly into any laboratory environment without compromising functionality. With features like smart qPCR workflow and RFID integration for TaqMan Array Plates, it streamlines data management and reduces manual inputs, enhancing laboratory productivity.

The system supports an extensive array of assays, emphasizing its versatility. User-friendly operation and intelligent workflow automation further optimize laboratory efficiency.

INTENDED FOR: (but not limited to the list)

• Infectious diseases
  COVID-19
  Monkeypox
  Herpes Zoster
  Herpes Simplex
• STD Diseases
  Syphilis
  AIDS
  Condyloma Acuminatum
• Genetic Diseases
• Molecular Biology Research

PACKING CONTENTS:

Intended Use
N96 Bacteria Genomic DNA Kit is based on our proprietary silica membrane purification technology, capable of quickly extracting and purifying bacteria genomic DNA. Combined with 96-well frits, 96-well collection plates and vacuum or centrifuge, kit would be fast, efficient and high-throughput to extract genomic DNA. Extracted DNA is ready to use in many downstream applications, including restriction digestion, PCR amplification, ligation and transformation, PCR, sequencing and library construction.

Features

• Suitable for vacuum or centrifuge.
• 96-well for high-throughput samples processing.
• High quality with reproducible yields.

Intended Use
N96 Plant Genomic DNA Kit is based on our proprietary silica membrane purification technology, capable of quickly extracting and purifying plant genomic DNA. Combined with 96-well frits, 96-well collection plates, and vacuum or centrifuge, the kit would be fast, efficient, and high-throughput to extract genomic DNA. Extracted DNA is ready to use in many downstream applications, including restriction digestion, PCR amplification, ligation and transformation, PCR, sequencing, and library construction.

Features

• Suitable for vacuum or centrifuge.
• 96-well for high-throughput samples processing.
• High quality with reproducible yields.

Intended Use
N96 Plasmid Miniprep Kit is based on our proprietary silica membrane purification technology capable of quickly extracting and purifying plasmid DNA. Combined with 96-well frits, 96-well collection plates, and vacuum or centrifuge, a kit would be fast, efficient, and high-throughput to extract plasmid DNA. Extracted DNA is ready to use in many downstream applications, including restriction digestion, PCR amplification, ligation and transformation, PCR, sequencing, and library construction.

Features

• Suitable for vacuum or centrifuge.
• 96-well for high-throughput samples processing.
• High quality with reproducible yields.

Intended Use
PCR DNA Purification Kit (Magnetic Beads) is designed for rapid and reliable purification of PCR Products. This kit can purify DNA fragments from direct PCR samples or samples with loading buffer. The purification rate of above 100 bp DNA fragments are up to 80%. The kit can obtain nucleic acid products with excellent purity after the steps of binding liquid, magnetic bead adsorption of DNA fragments, washing and elution. The purified PCR products is suitable for use in the most sensitive downstream applications, including PCR analysis, restriction enzyme digestion, sequencing.

Features

• The purified DNA has high quality and good integrity
• The kit can be used with nucleic acid extraction instruments to get large-scale and high-throughput extraction. It is simple, fast and efficient.