His-Tag Purification Prepacked Columns

( 0 out of 5 )
SKU: P-AC0101-51 / P-AC0101-55 / ACM050


His-Tag Purification prepacked columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni2+ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with Poly histidine tags. The Beingbio His prepacked columns provide a fast, convenient, and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.

Recombinant proteins with Poly histidine tags are retained due to the formation of complex compounds between their Poly histidine tags and immobilized Ni2+ ions. Impurity proteins without Poly histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

His-Tag Purification prepacked columns are available in 1 mL and 5 mL sizes.



  • Superior biocompatibility
  • Uncompromised biological activities
  • High purification yields
  • Moderate experimental conditions



  • Purification of recombinant proteins with Poly-histidine tags from various express systems, such as bacterial, insect, and mammal cells.
  • purification of His tagged membrane proteins to study lipid binding.
  • purification of His tagged spider toxin and variants to evaluate sodium channel inhibition.
SKU: P-AC0101-51 / P-AC0101-55 / ACM050 Category:


Cat. #DescriptionQty.
P-AC0101-51NI Copure FF Prepacked Columns, 1 mL5/Box
P-AC0101-55NI Copure FF Prepacked Columns, 5 mL5/Box
ACM050Ni-IDA Chromatographic Medium, Particle size 50 um, Pore Size 800 A250 mL/Vial

Note: His-Tag Purification prepacked columns and Ni-IDA chromatographic medium should be stored at 2-8 °C.

Product No

ACM050, P-AC0101-51, P-AC0101-55

Packing Size

250 mL/Vial, 5 pcs/pack

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Verified by MonsterInsights