INTENDED USE
CommaX ^TM G-25 desalting gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.

FEATURES

• Puncturable upper caps confer easy, tight connection with peristaltic pumps and syringes.
• Ease of use, no need for dedicated purification systems

INTENDED USE

CommaX^TM G-25 desalting prepacked columns take full advantages of LC purification systems to desalt bio macromolecules. Biocomma provides 1-mL and 5-mL G-25 desalting prepacked columns.

The products are prepacked, ready to use columns for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

FEATURES

• Direct connection with GE ÄKTA purifiers without any need for adapters.
• Peristaltic pumps and syringes can be used to assist purification.
• Two or more columns connected in series can handle large sample volumes.
• High desalting yield is achieved in minutes.

INTENDED USE

CommaX ^TM G-25 desalting spin columns works with high-speed centrifuges to process large samples in parallel in short time. They are very affordable and handy tools for protein desalting. Quickly desalt protein and biological samples for manual or automated liquid chromatography (LC) with BeingBio Spin Desalting columns, which offers excellent protein desalting and recovery in a centrifuge format.

Each desalting spin column includes: one collection tube, one adapter, one 12-mL prepacked chromatographic column, one upper cap, one bottom cap and two frits.

FEATURES

• Large sample volumes
• High desalting efficiency and recovery
• Timesaving

INTENDED USE

CommaX^TM His gravity flow columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni²⁺ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with poly-histidine tags.

Recombinant proteins with poly-histidine tags are retained due to the formation of complex compounds between their poly-histidine tags and immobilized Ni²⁺ ions. Impurity proteins without poly-histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

CommaX^TM His gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.

FEATURES

• Puncturable upper caps confer easy connection with peristaltic pumps and syringes
• Ease of use, no need for dedicated purification systems

APPLICATIONS

• Purification of recombinant proteins with poly-histidine tags from various express systems, such as bacterial, insect and mammal cells.

INTENDED USE

His-Tag Purification prepacked columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni²⁺ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with Poly histidine tags. The Beingbio His prepacked columns provide a fast, convenient, and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.

Recombinant proteins with Poly histidine tags are retained due to the formation of complex compounds between their Poly histidine tags and immobilized Ni²⁺ ions. Impurity proteins without Poly histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

His-Tag Purification prepacked columns are available in 1 mL and 5 mL sizes.

FEATURES

• Superior biocompatibility
• Uncompromised biological activities
• High purification yields
• Moderate experimental conditions

APPLICATIONS

• Purification of recombinant proteins with Poly-histidine tags from various express systems, such as bacterial, insect, and mammal cells.
• purification of His tagged membrane proteins to study lipid binding.
• purification of His tagged spider toxin and variants to evaluate sodium channel inhibition.

INTENDED USE
Protein A and Protein G can bind specifically to Fc regions of many mammalian immunoglobulins. It has been well-documented that the Fc binding characteristics of recombinant Protein A and Protein G are identical to their natural counterparts.

rProtein Prepacked Columns can purify immunoglobulins and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants, and other biological fluids.

FEATURES

• Innovative coupling technology, higher purification efficiency
• Better specific binding capacity
• For manual operation and ÄKTA systems