SPECIFICITY
Cylindrospermopsin residues can be detected by this assay. Common cyanotoxins which can be found in water samples were tested in the assay and their reactivity is listed in the table below.

Compound                                                  % Reactivity
Cylindrospermopsin                                       100%
7-Epi-Cylindrospermopsin                            153%
7-Deoxy-Cylindrospermopsin                      126%
Microcystin-LR                                                 <1%
Nodularin                                                          <1%

ASSAY PRINCIPLES
The Cylindrospermopsin Plate Kit is a competitive enzyme-labeled immunoassay. Cylindrospermopsin HRP enzyme conjugate is pipetted into the test wells followed by calibrators or samples. Cylindrospermopsin Antibody Solution is added into the test wells to initiate the reaction.

During the 45-minute incubation period, cylindrospermopsin from the sample and cylindrospermopsin HRP conjugate compete for binding to the cylindrospermopsin antibody. Following this incubation, the wells are washed to remove any unbound cylindrospermopsin or cylindrospermopsin HRP conjugate. After washing, a colorless substrate is added to the wells and any bound cylindrospermopsin HRP conjugate will convert the substrate to a blue color. Following a 45-minute incubation, the reaction is stopped and the amount of color in each well is measured. The color of the unknown sample is compared to the color of the calibrators and the cylindrospermopsin concentration of the sample is derived.

NOTE: Color is inversely proportional to cylindrospermopsin concentration.
Darker color = Lower concentration
Lighter color = Higher concentration