His Gravity Flow Columns
$199.00 – $299.00
INTENDED USE
CommaXTM His gravity flow columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni2+ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with poly-histidine tags.
Recombinant proteins with poly-histidine tags are retained due to the formation of complex compounds between their poly-histidine tags and immobilized Ni2+ ions. Impurity proteins without poly-histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.
CommaXTM His gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.
FEATURES
- Puncturable upper caps confer easy connection with peristaltic pumps and syringes
- Ease of use, no need for dedicated purification systems
APPLICATIONS
- Purification of recombinant proteins with poly-histidine tags from various express systems, such as bacterial, insect and mammal cells.
INTENDED USE
CommaXTM His gravity flow columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni2+ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with poly-histidine tags.
Recombinant proteins with poly-histidine tags are retained due to the formation of complex compounds between their poly-histidine tags and immobilized Ni2+ ions. Impurity proteins without poly-histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.
CommaXTM His gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.
FEATURES
- Puncturable upper caps confer easy connection with peristaltic pumps and syringes
- Ease of use, no need for dedicated purification systems
APPLICATIONS
- Purification of recombinant proteins with poly-histidine tags from various express systems, such as bacterial, insect and mammal cells.
ORDER INFORMATION:
| Cat. # | Description | Qty. |
|---|---|---|
| ACHIS06 | CommaXTM His Gravity Flow Columns, 1 mL/6 mL | 30/Box |
| ACHIS06L | CommaXTM His Gravity Flow Columns, 3 mL/6 mL, Long-Form | 20/Box |
| ACHIS12 | CommaXTM His Gravity Flow Columns, 12 mL | 20/Box |
Note: CommaXTM His gravity flow columns should be stored at 2-8 °C.
| Product No | ACHIS06, ACHIS06L, ACHIS12 |
|---|---|
| Packing Size | 20 pcs/pack, 30 pcs/pack |
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