His Gravity Flow Columns

INTENDED USE

CommaX^TM His gravity flow columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni²⁺ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with poly-histidine tags.

Recombinant proteins with poly-histidine tags are retained due to the formation of complex compounds between their poly-histidine tags and immobilized Ni²⁺ ions. Impurity proteins without poly-histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.

CommaX^TM His gravity flow columns can be operated by gravity flow. They offer higher purification performance if operated by positive pressure via peristaltic pumps or syringes.

FEATURES

• Puncturable upper caps confer easy connection with peristaltic pumps and syringes
• Ease of use, no need for dedicated purification systems

APPLICATIONS

• Purification of recombinant proteins with poly-histidine tags from various express systems, such as bacterial, insect and mammal cells.