INTENDED USE
His-Tag Purification prepacked columns are packed with highly cross-linked Poly (methyl methacrylate) beads which are coated with a propriety hydrophilic layer, then grafted with IDA ligands which donate binding sites to Ni2+ ions, forming an immobilized metal affinity chromatography (IMAC) medium for purifying recombinant proteins with Poly histidine tags. The Beingbio His prepacked columns provide a fast, convenient, and reproducible format for preparative purification of histidine-tagged (His-tag) recombinant proteins.
Recombinant proteins with Poly histidine tags are retained due to the formation of complex compounds between their Poly histidine tags and immobilized Ni2+ ions. Impurity proteins without Poly histidine tags are washed off. The target proteins are eluted with an imidazole buffer solution.
His-Tag Purification prepacked columns are available in 1 mL and 5 mL sizes.
FEATURES
APPLICATIONS
ORDER INFORMATION:
| Cat. # | Description | Qty. |
|---|---|---|
| P-AC0101-51 | NI Copure FF Prepacked Columns, 1 mL | 5/Box |
| P-AC0101-55 | NI Copure FF Prepacked Columns, 5 mL | 5/Box |
| ACM050 | Ni-IDA Chromatographic Medium, Particle size 50 um, Pore Size 800 A | 250 mL/Vial |
Note: His-Tag Purification prepacked columns and Ni-IDA chromatographic medium should be stored at 2-8 °C.
| Product No | ACM050, P-AC0101-51, P-AC0101-55 |
|---|---|
| Packing Size | 250 mL/Vial, 5 pcs/pack |
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