ASSAY PRINCIPLES
The Invertase kit is a competitive enzyme-labeled immunoassay. Invertase antibody and sample extracts/calibrators are pipetted into mixing wells and incubated for 30 minutes. The sample-antibody mixture is then pipetted into the antigen coated test wells to initiate the reaction. During the 30-minute incubation period, Invertase from the sample and Invertase on the coated strips compete for binding to Invertase antibody. Following this 30-minute incubation, the contents of the well are removed, and the wells are washed. GAR-HRP is then added to the wells incubated for 30 minutes. During this 30 minutes GAR-HRP binds to the Invertase antibody. Following this incubation, the contents of the well are removed, and the wells are washed to remove any unbound GAR-HRP. A clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 30-minute incubation, the reaction is stopped and amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the Invertase concentration of the samples is derived.